Chromatography and Its Applications [biological] by S. Dhanarasu

By S. Dhanarasu

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Fr G6 was applied to repeated column chromatographies (CC) over Sephadex LH-20 eluted with MeOH to afford isoorientin (Luteolin 6-C-glucoside) and luteolin 7-O-glucoside. Fr. G2 was subjected to column chromatography on Sephadex LH-20 using MeOH to give compound (2’’-O-transcaffeoylisoorientin. Fractionation of Fr. F by open CC on silica gel using EtOAc-MeOH-H2O (100:17:13) yielded subfractions F1-6 . Fr. , 2008). 40 Chromatography and Its Applications OH HO O OH OR3 O R2O OR1 HO OH R1 =H, R2=CH3, R3=trans caffeoyl R1 =R2=H, R3=trans caffeoyl R1 = trans caffeoyl, R2=R3=H O luteolin 6-C-(4’’-methyl-6’’-O-trans-caffeoylglucoide) luteolin 6-C-(6’’-O-trans-caffeoylglucoide) luteolin 6-C-(2’’-O-trans-caffeoylglucoide) R6 R1 O R5 R2 R4 O R3 R1 =O-Glc(6’’-p-hydroxybenzoyl) R2=R4=H, R3=R5=R6=OH R1 =R2=R4=R5=OCH3, R3=R6=OH luteolin 7-O-(6’’-p-benzoylglucoide) 4’, 5-dihyroxy-3, 3’, 6, 7tetramethoxyflavone luteolin artemetin isorhamnetin R1 =R3=R5=R6=OH, R2=R4=H R1 =R2=R4=R5=R6=OCH3, R3=OH R1 =R3=R4=R6=OH, R5=OCH3, R2=H OH HO OH O OH OH O O O OH OH O OH OH HO HO OH O HO HO OH isoorientin O lutelin-7-glycoside Isoflavonoids such as puerarin, daidzin and daidzein was isolated from Pueraria lobata (Willd) Ohwi roots.

Taxol (Paclitaxel), a diterpenoid isolated from Taxus brevifolia Nutt. , 2004). Taxol has been isolated from T. brevifolia using many different chromatographic techniquies and one of the way was described by Senihl et al. (1984) which employs normal phase chromatography columns for the separation procedures and includes multiple (seven) steps respectively as follows; 1. Extraction with alcohol and concentration, 2. Partition between water and dichloromethane, 3. Filtration chromatography, 4. Silica column chromatography, 5.

An aliquot of the CH2Cl2 extract was applied to a silica gel column eluted successively with CHCl3–MeOH (200:1–1:1 gradient system) to obtain 5 fractions. Fraction 2 was subjected to a silica gel column eluted with petroleum ether–EtOAc (10:1–1:1 gradient system) to afford six fractions, F21–F26. F21 was subjected to Sephadex LH-20 column chromatography (petroleum ether–CHCl3–MeOH, 2:1:1), then recrystallized to afford ganoderic acid DM. F22 was applied to a Sephadex LH-20 (petroleum ether–CHCl3– MeOH, 2:1:1), and then further purified by semipreparative HPLC (MeOH–H2O, 90:10) to afford ganodermanondiol and ganoderic acid T-Q.

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