Biological applications of magnetic resonance by R G Shulman

By R G Shulman

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H. (1975a). Biochemistry 14, and von H i p p e l , P. H. (1975b). Biochemistry 14, 1281-1296. McGhee, J. , 1297-1303. Marshall, Τ. , and Grunwald, E. (1969). J. Am. Chem. Soc. 91, 4541-4544. Patel, D. J. (1974). Biochemistry 13, Patel, D. J. (1975). Biochemistry 14, 2396-2402. Patel, D. J. (1976). Biopolymers 15, 533-558. Patel, D. J. (1977). Biopolymers 16, 1635/1656. Patel, D. , 3984-3989. and H i l b e r s , C. W. (1975). , and Tonelli, A. (1974). Biopolymers T. , Miles, 14, 2651-2656. Patel, D.

0. Two of P e preirradiation water where ditions: 1977, of the the conditions tRNA . saturated (c), from of phe yeast spectrum resonance away Demonstration and that Hoogsteen of the Ug complex been assigned by thio Ug in ring N3 proton to the N 7 atom C . W . 4 ppm et a l . , to N 1977). resonances The the more labile to be from with the is c o n c l u d e d that in limited. Assuming dependent on about sec" 100 the which is sec" measured 1 lower from termining the much less ent, it the state at 40°C and of Wong are G et rate an with 36°C) it [see E q .

Breaking are broken by monitoring by = k_x sociation if b a s e p a i r s can be characterized and to (k^) and followed at processes is related 1 be changes The making formation τ" can absorption tramolecular time temperature this 33 of in is e s t i m a t e d than required the for pK disappearance to formation Eq. (8a) be rate are C. W . H i l b e r s 34 Fig. 13. G1 tRNA y yeast perature jump melting Plot as of change experiments. temperatures line representing ature τ values lated to the the a function of l/k_i and of the relaxation temperature The vertical arrows individual transition.

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